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Calcium chloride transformation
Plasmid DNA can attach to LPS by being added to the cell solution together with CaCl2. Thus, when heat shock is applied, the negatively charged DNA ... ...(以下省略)
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Calcium Chloride imparts a positive charge to the cell wall. Since DNA is negatively charged molecule, the positive char...
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Heat shock the cells in a 42oC water bath for 45 to 60 seconds and return to ice for 1 minute. Add 1.2ml of LB broth to ...
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Plasmid DNA can attach to LPS by being added to the cell solution together with CaCl2. Thus, when heat shock is applied,...
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由 AY Chang 著作 · 被引用 189 次 — Calcium chloride heat-shock transformation is a powerful molecular biology technique use...
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Transfer the tubes to the heat block and incubate at 42°C for 45 s exactly. NOTE: Heat shock is a critical step. Do not ...
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由 M Rahimzadeh 著作 · 2016 · 被引用 68 次 — CaCl2 treatment followed by heat shock is the most common method for artific...
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由 A Asif 著作 · 2017 · 被引用 73 次 — Treatment with divalents or trivalents on ice is followed by treatment with elevat...
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cacl2 heat shock method 參考影音
繼續努力蒐集當中...